Abstracts Clinical Lymphoma, Myeloma & Leukemia September 2023 S280 of the MMP-2 gene in AML (n=173) and control (n=70) cases. Then, we did correlation analysis to find the potentially associated gene linked with the MMP-2 gene based on their expression levels using UALCAN database. Kaplan–Meier survival estimation was performed to evaluate the prognostic significance of MMP-2 using Kaplan Meier plotter. In addition, we assessed the DNA methylation status of MMP-2 gene using MEXPRESS database. Moreover, we also explored the molecular mechanisms of MMP-2 in AML by performing gene set enrichment analysis (GSEA) analysis. Finally, the relationship of MMP-2 expression with different functional states was studied using SANGER Box 3.0 database. Results: In our analyses, MMP-2 mRNA was significantly overexpressed in AML (n=173) cohorts as compared with normal cases (n=70) and closely associated with poor overall survival (P <0.05). In correlation analysis MMP-2 gene was positively correlated with the WT-1 gene, followed by CPA3, IGFBP2, FGFRL1, genes (PCC <0.65). Furthermore, a lower methylation level of MMP-2 gene was observed in AML compared to normal cases, which were negatively associated with MMP-2 gene expression. Gene enrichment analysis revealed MMP-2 gene and its positively associated gene were involved in biological processes such as angiogenesis, blood vessel maturation, and leukocyte transendothelial migration pathway. Notably, the MMP-2 mRNA expression was negatively linked with cell cycle and proliferation while positively associated with differentiation invasion and metastasis in AML. Conclusions: In this study, we found that MMP-2 plays a significant role in AML progression and may serve as a potential prognostic biomarker and therapeutic target for the effective management of AML. Keywords: acute myeloid leukemia, MMP-2, gene expression, methylation, biomarker AML-233 Incidence of Oral Manifestations in Patients With Hematological Malignancy Mai Mostafa Aly MD1, Merit Rafeek Botros MD1, Nabila Faiek Ameen MD2, Mina Wassem Shokr MD3, Mohammed Nahed Attia Mohammed PhD3 1Clinical Hematology Unit, Internal Medicine Department, Faculty of Medicine, Assiut, Egypt. 2Internal Medicine Department, Faculty of Medicine, Assiut University, Assiut, Egypt. 3Oral & Maxillofacial Surgery Department, Faculty of Dentistry, Assiut, Egypt Oral health status and oral manifestations can give us some clues about the incidence and progression of the disease process in patients with hematological malignancy. Patients may suffer from oral ulcers, bleeding, and infections that might affect hospital stay, treatment cost, and patient prognosis. The oral manifestations of patients with hematological malignancy can occur due to the disease itself or because of the current medication during treatment. This study aims to evaluate the incidence and types of oral manifestation found in patients diagnosed with hematological malignancy. A prospective cross-sectional study (NCT05758207) included all newly diagnosed patients older than 18 years with hematological malignancy through 2023 in Clinical Hematology Unit, Assiut University Hospitals. We examined 55 cases diagnosed as acute myeloid leukemia (AML; n=25, 46%), acute lymphoblastic leukemia (B-ALL; n=20, 36%), bi-phenotypic acute leukemia (n=5;9%), and myelodysplastic syndrome with an excess blast (MDS-EB; n=5, 9%). The median (range) age is 40 (20-50) years, and 60% (n=33) were females. Blood count median (range) WBC 50x109/L (3.2-500), ANC 0.3x109/L (0.2-0.4), hemoglobin 6.8g/dl (5.2-9.9), platelet count 82x109/L (75-106), abnormal cells 19% (10-95). Nine (16%) cases are FLT3 positive, and 9% (n=5) are BCR-ABL positive. Almost half (n=30; 55%) of the included cases treated with 3&7 protocol as induction chemotherapy, (n=15; 27%) received high dose Ara-c (HIDAC) as consolidation, (n=10; 18%) received HYPER-CVAD protocol as induction chemotherapy and (n=5; 9%) received tyrosine kinasebased regimen (BCR-ABL positive ALL). The median (range) overall survival of the studied cohort was 6.9 (0.23-12) months. The dental examination using a standardized assessment oral clinic sheet at diagnosis showed 50 cases (91%) had gingival hyperplasia (generalized n=35, localized n=15); buccal cavity infection (n=15; 27%) where 5 cases had a fungal infection, buccal mucosa and lingual ulceration (n=15; 27%), spontaneous gingival bleeding (n=10; 18%) and xerostomia (n=10; 18%). In conclusion, this study showed the types and frequency of oral conditions observed in hematological malignancy cases. Professionally planned oral hygiene protocols, regular follow-ups, and oral microbial surveillance are highly recommended to avoid complications. We plan to evaluate a larger population to establish a correlation between various hematological malignancies and oral complications. Keywords: AML, gingival hyperplasia, buccal cavity infection, buccal ulceration, hematological malignancy AML-240 Understanding Physicians’ Approach to Diagnosis and Treatment of Acute Myeloid Leukemia Disease in Türkiye: Turkish Hematology Networking Group Survey Birol Güvenç MD1, Mehmet Ali Özcan MD2, Mehmet Sönmez MD3, Meltem Aylı MD4, Sevgi Kalayoğlu Beşişik MD5 1Çukurova University Faculty of Medicine, Division of Hematology, Adana, Turkey. 2Dokuz Eylül University Faculty of Medicine, Division of Hematology, Izmir, Turkey. 3Karadeniz Technical University Faculty of Medicine, Division of Hematology, Trabzon, Turkey. 4SBU Gülhane Training and Research Hospital, Division of Hematology, Ankara, Turkey. 5Istanbul University, İstanbul Faculty of Medicine, Department of Hematology, Istanbul, Turkey Background and Objectives: This study was done by the Turkish Hematology Networking Group (THNG) to analyze the characteristics of hematologists, their diagnosis, and treatment approaches for acute myeloid leukemia (AML) patients in Turkey. A better understanding of AML diagnosis and treatment approaches in the country, as well as the characteristics of the average AML patient, is essential for improving patient care. Methods: A survey was conducted among hematologists, collecting data on demographics, general information, diagnostic methods, FLT3
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